ABSTRACT
Danggui Liuhuang Tang， as one of classical traditional Chinese medicine prescriptions， has been used by major medical experts in clinic since Jin and Yuan dynasties. After review and summarization of relevant literatures on the pharmacological effects and clinical application of Danggui Liuhuang Tang， it was found that Danggui Liuhuang Tang has a wide range of pharmacological activities， and exerts its anti-inflammatory and anti-insulin resistance effects mainly by inhibiting the production of inflammatory factors， such as tumor necrosis factor and interleukin and activating related pathways. In addition， Danggui Liuhuang Tang inhibits the occurrence and development of hepatic fibrosis by attenuating proinflammatory signaling and extracellular matrix accumulation with multiple components， multiple targets， and multiple pathways. Danggui Liuhuang Tang has been widely used in sweat syndromes， with an obvious effect in the treatment of thyroid diseases， diabetes， respiratory tract and other diseases. This paper reviews and summarizes the pharmacological effects and clinical application of Danggui Liuhuang Tang，in an attempt to provide some valuable clues for the subsequent development of Danggui Liuhuang Tang.
ABSTRACT
Objectives To provide theoretical basis for the clinical rational using of drugs, biochemical characteristics of the liver injuries induced by the atorvastatin, simvastatin and lovastatin were analyzed and compared by replicating the rats model of liver injuries induced by statins, Methods 80 SPF SD rats (8 weeks of age), half male and half female, were divided into four groups randomly: control group, simvastatin group, lovastatin group, atorvastatin group. Human equivalent doses were administered to the latter three groups of rats which were sacrificed to draw blood on the 10 th, 35 th and 55 th day respectively (via the femoral artery) for testing liver function index, including total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), aspartateaminotransferase (AST), alanineaminotransferase (ALT), alkalinephosphatase (ALP). Results (1) The increases in TBIL, DBIL, IBIL for treatment group in comparison with control group had statistical significance (P＜0.01).2) At the 55 th day of administration, there was a significant statistical difference between the simvastatin group and the lovastatin group in AST (P＜0.05);3) Meanwhile, there was great statistical difference between the atorvastatin group and lovastatin group in ALP (P＜0.01). Conclusion The rats in the three experimental groups suffered from minor to moderate liver injuries, most of which being cholestasis type. It is speculated that this kind of liver injuries are closely related to the obstacle of transfering bile.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of water extract of Zuojin Pill ([characters: see text], ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism.</p><p><b>METHODS</b>Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9.</p><p><b>RESULTS</b>ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation.</p><p><b>CONCLUSIONS</b>ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.</p>